The former Kangaroo was due to play three quarters in the game against EFL side Vermont, but was withdrawn from the match at half time.
Wells was signed by the Pies in free agency to a three-year deal and has endured calf issues for the majority of his career.
Collingwood coach Nathan Buckley played down the injury setback.
“Wellsy was scheduled for three (quarters), got a kick in the calf,” Buckley said.
“We didn’t take any risks, pulled him after two quarters. But he’ll train this week and continue his step up in the workrate and load. We had four other listed players that played and got through really well.”
Wells is doubtful to play in Collingwood’s next JLT Series fixture against Fremantle in Mandurah on March 4. However, Buckley said captain Scott Pendlebury and Adam Treloar will likely feature in the match.
“The players that were rested for this game will play in the next two,” Buckley said.
“If there’s anyone else we need to manage, we will. But it’s pretty much trying to get our best mix together, build the chemistry and continue to build momentum.”