Collingwood veteran Scott Pendlebury's scans have confirmed a hamstring injury and a knee bursa.
The 38-year-old is expected to miss between two to three weeks in a major blow to the Pies' finals hopes.
Collingwood defender Brayden Maynard sent a shock through the Magpies camp after he appeared to injure his knee during a training incident on Saturday.
It has been revealed Maynard suffered a grade one MCL strain.
The tough defender was nearly ready to return to the senior side for the club's Friday night match against Brisbane.
Instead, the Pies decided to take a tactful approach and keep him rested for the Wildcard Round.
However, his availability is in serious jeopardy after he re-injured his knee during a tackle.
Maynard originally injured his knee during the club's Round 20 win over Adelaide.
Collingwood has a number of players under an injury cloud heading into next weekend's do-or-die clash.
Midfielder Patrick Lipinski, like Maynard, had a chance to return against the Lions but was rested to give him more time to recover from a calf injury.
Defender Isaac Quaynor likewise was given another week to recover from a glute/hip injury, with the 26-year-old a likely test for the final.
Wingman Harry Perryman is unlikely to be ready in time for the Wildcard Round clash as he recovers from a hamstring injury.
