Rioli got a knock to the knee during the second quarter, while Puopolo suffered a hamstring injury during the third term.
“We had a really challenging day on the injury front,” Hawthorn’s Director of High Performance Andrew Russell told hawthornfc.com.au.
“Cyril has a moderate grade medial injury.
“It is on the same side as his PCL last year… but it’s not related at all.
“It looks like it’s probably in the five to six week range at this stage.
Puopolo’s injury wasn’t as bad as first thought, although he’ll still miss a fair chuck of time.
“He’s probably pulled up a fraction better than we thought he would have,” Russell added.
“He’s got a decent hamstring strain and he’s going to miss four to six weeks.”
Hawthorn veteran Shaun Burgoyne will be unavailable this weekend, although he could return from his hamstring injury as early as round six.