McNiece joined the Bombers through through the Next Generation Academy in 2016 as a Category B rookie, playing 15 games for the club.
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Houlahan was drafted with pick No.49 in the 2017 national draft but failed to register a season game.
“On behalf of the club, I would like to thank Ben and Jordan for their efforts during their careers at Essendon,” general manager of football Dan Richardson told the club website.
“Ben displayed great dedication to the program, he was a popular player amongst the group and brought great leadership to our younger players.
“Although Jordan was unable to make his debut for the club, he always maintained professional standards and showed commitment in his approach to developing his football career.
“We wish both Ben and Jordan all the best in their future endeavours.”
Bombers list changes for 2020
Delisted – Ben McNiece and Jordan Houlahan