The ex-Docker has played every game for Collingwood since coming into the side round six, and was forced to missing training with his teammates.
Mayne is confident he will get up for Saturday night’s clash at the SCG, but his fitness is a concern for Nathan Buckley’s team amid a growing injury crisis.
Forward Brody Mihocek was walking laps on Tuesday after hurting his ankle last weekend against the Tigers, while Jeremy Howe was held to light training duties after recovering from a concussion during last Saturday’s clash.
Howe will look to prove his fitness during Thursday’s main training session.
Veterans Tyson Golsack (ACL) and Ben Reid (knees) joined in Tuesday’s session as they look to make a return through the VFL.