MELBOURNE, AUSTRALIA - JULY 26: Adam Treloar of the Magpies and team mates look dejected after the round 19 AFL match between the Collingwood Magpies and the Richmond Tigers at Melbourne Cricket Ground on July 26, 2019 in Melbourne, Australia. (Photo by Matt King/Getty Images)

Collingwood star Adam Treloar is set to miss his side's blockbuster Round 2 clash against Richmond.

The midfielder missed his side's Round 1 win over the Western Bulldogs due to a hamstring issue and suffered a setback of the same injury while training in isolation.

Upon returning to club training last week, Treloar experienced tightness in one of his calves.

Collingwood coach Nathan Buckley all but ruled out Treloar for the season reopener against Richmond at the MCG on Thursday June 11, saying the club will prepare to go in without the 27-year old.

"Treloar came back in and had done a fair block of work," he told reporters on Wednesday afternoon.

"He handled the work over the break really well. He just felt a bit tight in one of his calves. It’s really a low grade strain and is already bouncing around the joint and finding his way back to full fitness.

"We wouldn’t be anticipating that (Treloar) would be available for round two.

"We’re still looking at what our squad will be. There’s been a lot of water that’s gone under the bridge since we played the Bulldogs."