Wingard suffered a hamstring injury during the Power’s loss to Melbourne on the weekend, with the recovery time generally around three weeks.
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Hartlett suffered a calf injury prior to Port’s win over Brisbane a fortnight ago and will not recover in time for the end of the season.
Meanwhile, Frampton has been battling a groin injury for several weeks.
“It’s not worth risking Billy or Hamish given the nature of their injuries,” Port Adelaide high performance manager Darren Burgess said.
“We’ll have scans for Chad come through tomorrow, but I’d expect him to fall into the same boat.”